Publications
and Scientific Papers
Introduction
In vitro transcription (IVT) reaction for synthesis of mRNA is commonly performed by adding T7 RNA polymerase (RNAP) and a DNA template. Although the reaction is very efficient, T7 RNAP generates additional immunostimulatory dsRNA impurities – abortive, antisense, and loop-back dsRNAs during the transcription initiation and termination.